Comparative proteomic analysis of mesenchymal stem cells derived from human bone marrow,umbilical cord,and placenta: Implication in the migration |
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| Authors: | Guo Li Xiao‐ai Zhang Hua Wang Xin Wang Chun‐ling Meng Chu‐yan Chan David Tai Wai Yew Kam Sze Tsang Karen Li Sau‐na Tsai Sai‐ming Ngai Zhong Chao Han Marie Chia‐mi Lin Ming‐liang He Hsiang‐fu Kung Professor |
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| Institution: | 1. Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, P.R. China;2. Department of Anatomy, The Chinese University of Hong Kong, Hong Kong, P.R. China;3. Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong, P.R. China;4. Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Hong Kong, P.R. China;5. Department of Pediatrics, The Chinese University of Hong Kong, Hong Kong, P.R. China;6. Department of Biology, The Chinese University of Hong Kong, Hong Kong, P.R. China;7. Institute of Hematology, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, P.R. China;8. Department of Chemistry, The University of Hong Kong, Hong Kong, P.R. China;9. State Key Laboratory in Oncology in South China, Cancer Center, Sun Yat‐Sen University, Guangzhou, P.R. China |
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| Abstract: | Umbilical cord (UC) and placenta (P) have been suggested as alternatives to bone marrow (BM) as sources of mesenchymal stem cells (MSC) for cell therapy, with both UC‐ and P‐MSC possess immunophenotypic and functional characteristics similar to BM‐MSC. However, their migration capacity, which is indispensable during tissue regeneration process, is unclear. Under defined conditions, the migration capacity of BM‐ and P‐MSC was found 5.9‐ and 3.2‐folds higher than that of UC‐MSC, respectively. By the use of 2‐DE and combined MS and MS/MS analysis, six differentially expressed proteins were identified among these MSC samples, with five of them known to be involved in cell migration as migration enhancing or inhibiting proteins. Consistent with their migration capacity, the levels of migration enhancing proteins including cathepsin B, cathepsin D and prohibitin,were significantly lower in UC‐MSC when compared with those in BM‐ and P‐MSC. For the migration inhibiting proteins such as plasminogen activator inhibitor‐1 (PAI‐1) and manganese superoxide dismutase, higher expression was found in the UC‐MSC. We also showed that the overexpression of the PAI‐1 impaired the migration capacity of BM‐ and P‐MSC while silencing of PAI‐1 enhanced the migration capacity of UC‐MSC. Our study indicates that PAI‐1 and other migration‐related proteins are pivotal in governing the migration capacity of MSC. |
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| Keywords: | Bone marrow Mesenchymal stem cells Migration Placenta Umbilical cord |
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