首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Adaptation of the rat cardiac proteome in response to intensity‐controlled endurance exercise
Authors:Jatin G Burniston Dr
Institution:Research Institute for Sport and Exercise Sciences (RISES) and Institute for Health Research (IHR), Liverpool John Moores University, Liverpool, UK
Abstract:Endurance training improves cardiac function and protects against heart disease. The rodent intensity‐controlled running model replicates endurance exercise in humans and can be used to investigate molecular adaptations in the heart. Rats (n = 6, 280 ± 3 g) performed exercise tests to measure their peak oxygen uptake (equation image ) and training was prescribed at 70–75% equation image for 30 min, 4 days/wk. Hearts were isolated 4 h after a final equation image test and left ventricle proteomes compared to weight‐matched control animals (n = 6, 330 ± 2 g) using differential analysis of 2‐D gels. Proteins were identified by searching MS and MS/MS spectra against Swiss‐Prot using MASCOT (www.matrixscience.com). Average equation image increased 23% (p = 0.008) over the 6‐week regimen and 23 gel spots differed (p<0.05) between exercised and control hearts. Expression of myofibrillar proteins (e.g. α‐myosin heavy chain and cardiac α‐actin) and proteins associated with fatty acid metabolism (e.g. heart fatty acid binding protein, acetyl coenzyme A dehydrogenase and mitochondrial thioesterase‐1) increased. In addition, this work discovered a novel increase in phosphorylation of heat shock protein 20 at serine 16. Previously this modification has been associated with improved cardiomyocyte contractility and protection against apoptosis.
Keywords:2‐D gel electrophoresis  Animal model  Exercise  Heart  HSP20 heat shock proteins
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号