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Proteomic analysis of a filamentous fungal endophyte using EST datasets |
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| Authors: | Shalome A. Bassett Judy J. Bond Fiona Y. S. Kwan Alan F. McCulloch Paul A. Haynes Richard D. Johnson Gregory T. Bryan Dr. T. William Jordan |
| Affiliation: | 1. AgResearch Ltd., Grasslands Research Centre, Palmerston North, New Zealand;2. Centre for Biodiscovery and School of Biological Sciences, Victoria University of Wellington, New Zealand;3. AgResearch Ltd., Proteomics Laboratory, Victoria University of Wellington, New Zealand;4. AgResearch Ltd., Invermay Agricultural Centre, Mosgiel, New Zealand;5. Department of Biochemistry and Molecular Biophysics, Bio5 Institute, University of Arizona, Tucson, AZ, USA;6. Department of Chemistry and Biomolecular Sciences, Macquarie University, North Ryde, New South Wales, Australia |
| Abstract: | Proteomic analysis of many species of fungi, particularly filamentous fungi, is difficult due to the lack of publicly available genome sequence data and the problems associated with cross‐species comparisons. Furthermore, the detection of fungal proteins in biological systems where there are a greater number of proteins present from other eukaryote species provides additional challenges. We present an EST‐based approach for identifying proteins from a fungal endophyte of temperate grasses and demonstrate that this method is well suited for fungi with minimal sequence data. |
| Keywords: | Endophyte Neotyphodium lolii Perennial ryegrass Symbiosis Two‐dimensional gel electrophoresis |