Nanoscale elongating control of the self‐assembled protein filament with the cysteine‐introduced building blocks |
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Authors: | Kengo Usui Tei Maki Fuyu Ito Atsushi Suenaga Satoru Kidoaki Masayoshi Itoh Makoto Taiji Takehisa Matsuda Yoshihide Hayashizaki Harukazu Suzuki |
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Affiliation: | 1. CREST, Japan Science and Technology Agency, Kawaguchi, Saitama 332‐0012, Japan;2. Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), Tsurumi‐ku, Yokohama 230‐0045, Japan;3. Genome Science Laboratory, RIKEN, Hirosawa, Wako 351‐0198, Japan;4. Division of Biomolecular Chemistry, Kyushu University, Fukuoka 812‐8581, Japan;5. Computational and Experimental System Biology Group, RIKEN Genomic Sciences Center, Tsurumi, Yokohama, Kanagawa 230‐0046, Japan;6. Genome Biotechnology Laboratory, Kanazawa Institute of Technology, Ishikawa 924‐0838, Japan |
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Abstract: | Self‐assembly of artificially designed proteins is extremely desirable for nanomaterials. Here we show a novel strategy for the creation of self‐assembling proteins, named “Nanolego.” Nanolego consists of “structural elements” of a structurally stable symmetrical homo‐oligomeric protein and “binding elements,” which are multiple heterointeraction proteins with relatively weak affinity. We have established two key technologies for Nanolego, a stabilization method and a method for terminating the self‐assembly process. The stabilization method is mediated by disulfide bonds between Cysteine‐residues incorporated into the binding elements, and the termination method uses “capping Nanolegos,” in which some of the binding elements in the Nanolego are absent for the self‐assembled ends. With these technologies, we successfully constructed timing‐controlled and size‐regulated filament‐shape complexes via Nanolego self‐assembly. The Nanolego concept and these technologies should pave the way for regulated nanoarchitecture using designed proteins. |
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Keywords: | self‐assembly nanofilament protein design molecular modeling protein nanomaterial |
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