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Comparison of two combinations of cyanine dyes for prelabelling and gel electrophoresis |
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| Authors: | Nikos Tsolakos Tanasit Techanukul Amanda Wallington Yuan Zhao Christopher Jones Judit Nagy Jun X. Wheeler Dr. |
| Affiliation: | 1. Laboratory for Molecular Structure, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, UK;2. Institute of Biomedical Engineering, Imperial College London, Bessemer Building, South Kensington, London, UK;3. Biotherapeutics, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, UK |
| Abstract: | We report the use of IC‐OSu ethyl‐Cy3 and ethyl‐Cy5 N‐hydroxysuccinimide ester (NHS) cyanine dyes, which have similar chemical properties as the CyDye? DIGE fluor minimal dyes for pre‐electrophoresis labelling. Multiple sample analyses in different laboratories indicate that the use of IC‐OSu ethyl‐Cy3 and ethyl‐Cy5 NHS ester cyanine dyes produces equivalent results to those obtained with DIGE CyDyes, and allows sample multiplexing and accurate quantitation for differential proteome analysis. |
| Keywords: | Cyanine dyes Fluorescence labelling Multiplexing Two‐dimensional differential in‐gel electrophoresis |