POSTMan (POST‐translational modification analysis), a software application for PTM discovery |
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| Authors: | Magnus Ø. Arntzen Christoffer Leif Osland Christopher Rasch‐Olsen Raa Reidun Kopperud Stein‐Ove Døskeland Aurélia E. Lewis Clive S. D'Santos Dr. |
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| Affiliation: | 1. PROBE Proteomic Platform, Department of Biomedicine, University of Bergen, Bergen, Norway;2. Stix AS, Midtunveien, Nesttun, Norway;3. Department of Biomedicine, University of Bergen, Bergen, Norway |
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| Abstract: | Post‐translationally modified peptides present in low concentrations are often not selected for CID, resulting in no sequence information for these peptides. We have developed a software POSTMan (POST‐translational Modification analysis) allowing post‐translationally modified peptides to be targeted for fragmentation. The software aligns LC‐MS runs (MS1 data) between individual runs or within a single run and isolates pairs of peptides which differ by a user defined mass difference (post‐translationally modified peptides). The method was validated for acetylated peptides and allowed an assessment of even the basal protein phosphorylation of phenylalanine hydroxylase (PHA) in intact cells. |
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| Keywords: | Alignment Comparative LC‐MS Post‐translational modifications Proteomic software |
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