Proteomic analysis of the secretome of human umbilical vein endothelial cells using a combination of free‐flow electrophoresis and nanoflow LC‐MS/MS |
| |
| Authors: | David Guillen Tunica Xiaoke Yin Anissa Sidibe Christin Stegemann Mikkel Nissum Lingfang Zeng Merce Brunet Manuel Mayr |
| |
| Affiliation: | 1. Pharmacology Laboratory (CDB), Hospital Clinic, IDIBAPS, Barcelona University, CIBERehd, Barcelona, Spain;2. King's BHF Centre, King's College London, London, UK;3. BD Diagnostics, Martinsried, Munich, Germany |
| |
| Abstract: | Human umbilical vein endothelial cells are the most widely used in vitro model for endothelial cells. Their secreted proteins, however, have not been comprehensively analysed so far. In this study, we accomplished to map the secretome of human umbilical vein endothelial cells by combining free‐flow electrophoresis with nanoflow LC‐MS/MS. This comprehensive analysis provides a basis for future comparative studies of protein secretion by endothelial cells in response to cardiovascular risk factors and is available on our website http://www.vascular‐proteomics.com . |
| |
| Keywords: | Atherosclerosis Cell biology Extracellular proteins Protease inhibitors Proteomic methods Secreted proteins |
|
|
