Multiplexed LC‐MS/MS analysis of horse plasma proteins to study doping in sport |
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Authors: | Chris Barton Paul Beck Richard Kay Phil Teale Jane Roberts |
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Institution: | HFL Sport Science, Quotient Bioresearch Ltd., Fordham, Cambridgeshire, UK |
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Abstract: | The development of protein biomarkers for the indirect detection of doping in horse is a potential solution to doping threats such as gene and protein doping. A method for biomarker candidate discovery in horse plasma is presented using targeted analysis of proteotypic peptides from horse proteins. These peptides were first identified in a novel list of the abundant proteins in horse plasma. To monitor these peptides, an LC‐MS/MS method using multiple reaction monitoring was developed to study the quantity of 49 proteins in horse plasma in a single run. The method was optimised and validated, and then applied to a population of race‐horses to study protein variance within a population. The method was finally applied to longitudinal time courses of horse plasma collected after administration of an anabolic steroid to demonstrate utility for hypothesis‐driven discovery of doping biomarker candidates. |
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Keywords: | Doping MS/MS Plasma proteome Quantitative analysis |
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