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Proteomics of mouse liver microsomes: Performance of different protein separation workflows for LC‐MS/MS |
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| Authors: | Victor G Zgoda Sergei A Moshkovskii Elena A Ponomarenko Timofey V Andreewski Arthur T Kopylov Olga V Tikhonova Stanislav A Melnik Andrei V Lisitsa Alexander I Archakov |
| Institution: | Institute of Biomedical Chemistry, Moscow, Russia |
| Abstract: | The mouse liver microsome proteome was investigated using ion trap MS combined with three separation workflows including SDS‐PAGE followed by reverse‐phase LC of in‐gel protein digestions (519 proteins identified); 2‐D LC of protein digestion (1410 proteins); whole protein separation on mRP heat‐stable column followed by 2‐D LC of protein digestions from each fraction (3‐D LC; 3703 proteins). The higher number of proteins identified in the workflow corresponded to the lesser percentage of run‐to‐run reproducibility. Gel‐based method yielded a number of predicted membrane proteins similar to LC‐based workflows. |
| Keywords: | Animal Proteomics Ion trap LC‐MS/MS Liver microsomes Membrane protein Organelle proteomics |