Improvement of peptide vectors for gene delivery with active targeting profiles for phosphatidylserine |
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| Authors: | Shinichi Kuriyama Yasushi Taguchi Kanako Nishimura Kazutoshi Yanagibashi Yoshiki Katayama Takuro Niidoime |
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| Institution: | 1. Pharmaceutical Research Center, Mochida Pharmaceutical Co., Ltd., Gotemba 412‐8524, Japan;2. Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 819‐0395, Japan;3. Center for Future Chemistry, Kyushu University, Fukuoka 819‐0395, Japan;4. PRESTO, Japan Science and Technology Corporation, Kawaguchi 332‐0012, Japan |
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| Abstract: | A cationic peptide, Td3701, which was derived from factor VIII that has affinity with phosphatidylserine (PS), showed efficient transfection ability for cells that express PS on the cell surface. PS is exposed on tumor cell surfaces therefore we have focused on PS as the target molecule for tumor specific gene delivery. In this article, to improve transfection efficiency and specificity in targeting tumor cells, some amino acid residues of Td3701 were replaced. The resulting peptide, Td3717, shows higher transfection efficiency (more than 30 times that of Td3701). The transfection efficiency was dependent on the amount of PS on the cell surface, suggesting that Td3717 bound with plasmid DNA could recognize PS on the cell surface. Td3717 is expected to be useful as an efficient gene carrier molecule specific to PS‐presenting tumor cells. Copyright © 2008 European Peptide Society and John Wiley & Sons, Ltd. |
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| Keywords: | α ‐helix peptide gene delivery phosphatidylserine targeting transfection |
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