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MiR-138 downregulates miRNA processing in HeLa cells by targeting RMND5A and decreasing Exportin-5 stability |
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| Authors: | Jie Li Ying Chen Xingliang Qin Junzhi Wen Hongmei Ding Wei Xia Shaohua Li Xueting Su Wei Wang Hui Li Qiang Zhao Tao Fang Lianghu Qu Ningsheng Shao |
| Institution: | 1.Department of Biochemistry and Molecular Biology, Beijing Institute of Basic Medical Sciences, Beijing 100850, China and 2.Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Sun Yat-sen University, Guangzhou, 510275, China |
| Abstract: | MicroRNAs (miRNAs) are a class of non-coding small RNAs that consist of ∼22 nt and are involved in several biological processes by regulating target gene expression. MiR-138 has many biological functions and is often downregulated in cancers. Our results showed that overexpression of miR-138 downregulated target RMND5A (required for meiotic nuclear division 5 homolog A) and reduced Exportin-5 stability, which results in decreased levels of pre-miRNA nuclear export in HeLa cells. We also found that miR-138 could significantly inhibit HeLa cell migration by targeting RMND5A. Our study therefore identifies miR-138–RMND5A–Exportin-5 as a previously unknown miRNA processing regulatory pathway in HeLa cells. |
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