Insulin stimulates turnover of phosphatidylcholine in rat adipocytes |
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Authors: | S. Lance Macaulay Richard G. Larkins |
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Affiliation: | (1) CSIRO Division of Biomolecular Engineering, 343 Royal Pde., 3052 Parkville, Victoria, Australia;(2) Department of Medicine, Royal Melbourne Hospital, University of Melbourne, Grattan Street 343 Royal Pde., 3050 Parkville, Australia |
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Abstract: | The present study investigated the effect of insulin on phosphatidylcholine turnover in rat adipocytes labelled to equilibrium with [14C]-choline. Insulin induced a rapid turnover of this major phospholipid that was maximal by 1 min and transient in nature. Following a 1 min stimulation of the cells with insulin at a maximally effective concentration (7 nM), a 4–6% decrease in the percentage of total cellular choline associated with this phospholipid was observed. This reflected a significant transient increase in the percentage of total cellular choline associated with phosphorylcholine, which together with diacylglycerol are the phospholipase C cleavage products of phosphatidylcholine. These effects were observed over a physiological range of insulin concentrations. No effect of insulin on any other choline phospholipid or metabolite (sphingomyelin, lysophophatidylcholine, glycerophosphocholine or choline) was seen. These results suggest that insulin stimulates a phospholipase C-mediated turnover of phosphatidylcholine in rat adipocytes. The rapid nature of this turnover suggests a potential role in signal transduction. |
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Keywords: | phosphatidylcholine phospholipase C insulin cell signalling (rat adipocytes) |
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