Studies on the isolation,growth and maintenance of Malassezia pachydermatis |
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| Authors: | Rodolfo Lorenzini Flavia de Bernardis |
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| Affiliation: | (1) Department of Veterinary Medicine, Istituto Superiore di Sanità, 00161 Rome, Italy;(2) Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, 00161 Rome, Italy |
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| Abstract: | Results related to the isolation, cultivation, culture and maintenance of the opportunistic pathogen Malassezia pachydermatis are reported. A dextrose nutrient medium with 1.5% yeast extract turned out to be the most favourable medium for its development. It permitted identification in 24 hours and maintenance of isolates for three months without subculturing. Addition of Tween 80 (1%) significantly enhanced the isolation of this yeast from clinical materials. |
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