Roles of histone acetylation modification in basal and inducible expression of hsp26 gene in D. melanogaster |
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Authors: | Yanmei Zhao Jun Lu Hui Sun Xia Chen Baiqu Huang |
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Institution: | (1) Institute of Nautical Medicine, Nantong University (former Nantong Medical College), Nantong, 226001, P.R. China;(2) Medical College, Nantong University (former Nantong Medical College), Nantong, 226001, P.R. China;(3) Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University (former Nantong Medical College), Nantong, 226001, P.R. China |
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Abstract: | Src-suppressed C kinase substrate (SSeCKS) plays a role in membrane-cytoskeletal remodeling to regulate mitogenesis, cell differentiation,
and motility. Previous study showed that lipopolysaccharide (LPS) induced a selective and strong expression of SSeCKS in the
vascular endothelial cells of lung. Here we show that LPS stimulation elevated expression of SSeCKS mRNA and protein in Rat
pulmonary microvascular endothelial cell (RPMVEC). LPS potentiated SSeCKS phosphorylation in a time- and dose-dependent manner,
and partly induced translocation of SSeCKS from the cytosol to the membrane after LPS challenge. The PKC inhibitor, Calphostin
C, significantly decreased LPS-induced phosphorylation of SSeCKS, inhibited SSeCKS translocation and actin cytoskeleton reorganization
after LPS challenge, suggesting that PKC may play a role in LPS-induced SSeCKS translocation and actin rearrangement. We conclude
that SSeCKS is located downstream of PKC and that SSeCKS and PKC are both necessary for LPS-induced stress fiber formation.
Chun Cheng and Haiou Liu are contributed equally to this work. |
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Keywords: | Rat pulmonary microvascular endothelial cell Lipopolysaccharide SSeCKS Actin |
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