Early events in rice somatic embryogenesis: Changes in cortical microtubule distribution and orientation |
| |
Authors: | B Q Huang X L Ye S Y Zee E C Yeung |
| |
Institution: | (1) Department of Botany, University of Hong Kong, Hong Kong;(2) South China Institute of Botany, Academia Sinica, 510650 Guangzhou, China;(3) Department of Biological Sciences, University of Calgary, T2N 1N4 Calgary, Alberta, Canada |
| |
Abstract: | Summary The changes in the microtubular pattern during the first few days of somatic embryo induction were studied using the rice
scutellum epithelium system. A clear difference in the distribution and orientation of the microtubules could be found as
early as 12 h after culturing. In the control treatment, the microtubules tended to congregate near the terminal part of the
cells and had an oblique to transverse orientation with respect to the long axis of the cells. In the hormone-treated explants,
the microtubules were more evenly distributed throughout the cells as compared to the control treatment. The microtubules
initially had an oblique orientation similar to the control. However, by 16 h, the majority of microtubules were aligned parallel
to the long axis of the cells. The scutellum epithelial cells in both the control- and hormone-treated explants divided periclinally
at about 48 h. In the control treatment, the scutellum epithelial cells gradually became vacuolated over time, while the hormone-treated
explants remained densely cytoplasmic. The fluorescence staining intensity gradually declined in the control, whereas a distinct
staining pattern of microtubules remained in the hormone-treated explants. |
| |
Keywords: | microtubules rice scutellum epithelium somatic embryogenesis |
本文献已被 SpringerLink 等数据库收录! |
|