Candida albicans ABG1 gene is involved in endocytosis |
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| Authors: | Verónica Veses Manuel Casanova Amelia Murgui Neil AR Gow & José P Martínez |
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| Institution: | The Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK;;Departamento de Microbiología y Ecologia, Facultad de Farmacia, Universitat de Valencia, Valencia, Spain;and;Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universitat de Valencia, Valencia, Spain |
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| Abstract: | The human fungal pathogen Candida albicans undergoes reversible morphogenetic transitions between yeast, hyphal and pseudohyphal forms. The fungal vacuole actively participates in differentiation processes and plays a key role supporting hyphal growth. The ABG1 gene of C. albicans encodes an essential protein located in the vacuolar membranes of both yeast and hyphae. Using fluorescence microscopy of a green fluorescent protein-tagged version of Abg1p, a fraction of the protein was detected in hyphal tips, not associated with vacuolar membranes. Live cell imaging of emerging germ tubes showed that Abg1p migrated to the polarized growth site and colocalized with endocytic vesicles. Phenotypic analysis of a methionine-regulated conditional mutant confirmed that Abg1p is involved in endocytosis. |
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| Keywords: | Candida albicans ABG1 gene endocytosis spitzenkörper vacuole |
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