Human breast epithelium in organ culture: Effect of hormones on growth and morphology |
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Authors: | Judy M Strum Elizabeth A Hillman |
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Institution: | (1) Department of Anatomy, University of Maryland, School of Medicine, 655 West Baltimore Street, 21201 Baltimore, Maryland;(2) Department of Pathology, University of Maryland, School of Medicine, 655 West Baltimore Street, 21201 Baltimore, Maryland |
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Abstract: | Summary Normal breast tissue from a 17-year-old girl was grown in organ culture for 3 weeks. A comparison was made between the effects
on the epithelium of a defined culture medium containing various combinations of hormones and a serum-supplemented medium
that has been used to successfully maintain other human tissues for 4 months routinely, and in some cases for up to 1 year.
After culture for 3 weeks the explants were exposed to 3H]thymidine and autoradiographs were prepared and evaluated in order to determine labeling indexes. The only serum-free defined
medium that permitted any significant survival or labeling of the cells contained insulin + hydroxycortisone + prolactin.
However, serum-supplemented medium alone gave an even higher labeling index, and this was elevated more in media containing
either progesterone or other combinations of hormones. Our study indicates that normal human breast (removed at the early
postovulatory stage of the menstrual cycle) can be maintained in a differentiated state for 12 days in serum-supplemented
media. By 2 weeks the cells had begun to migrate onto the surface of the explant. They then began to accumulate tonofilaments
so that after 3 weeks in culture nearly all of the cells contained tonofilaments. The one exception was found in breast tissue
cultured in the presence of human chorionic gonadotropin, where the cells maintained differentiated characteristics, despite
the fact that they contained many lysosomes.
This work was supported by Grant R01 CA20764 and in part by Contract N01-CP-95640 awarded by the National Cancer Institute. |
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Keywords: | human breast culture mammary autoradiography hormones |
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