Comparative proteomics of industrial lager yeast reveals differential expression of the cerevisiae and non-cerevisiae parts of their genomes |
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Authors: | Caesar Robert Palmfeldt Johan Gustafsson John S Pettersson Ellinor Hashemi Sayed Hossein Blomberg Anders |
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Institution: | 1. Department of Cell and Molecular Biology, Microbiology, G?teborg University, G?teborg, Sweden;2. Department of Mathematical Statistics, Chalmers University of Technology, G?teborg, Sweden;3. Department of Cell and Molecular Biology, Microbiology, G?teborg University, G?teborg, SwedenDepartment of Cell and Molecular Biology, Microbiology, G?teborg University, Medicinaregatan 9c, Box 462, SE‐405 30 G?teborg, Sweden Fax: +46‐31‐7732599=== |
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Abstract: | The proteomes of three industrial lager beer strains, CMBS33, OG2252 and A15, were analysed under standardised laboratory growth conditions. Protein spots in the 2-DE pattern of the lager strains were subjected to MS/MS to identify protein variants. We found the protein composition of the three lager strains to be qualitatively rather similar, while being substantially different from the Saccharomyces cerevisiae strain BY4742. Database searches using several fully sequenced genomes from the Saccharomyces genera indicated that the non-cerevisiae proteins in the 2-D pattern of lager strains were most closely related to S. bayanus. For many proteins the regulation of the bayanus-like protein and its cerevisiae counterpart varied in a strain-dependent manner, e.g. the bayanus-like form of Tdh3p was roughly eight-fold more abundant than the cerevisiae form in the OG2252 strain. We also found differential regulation of cerevisiae- and bayanus-like proteins during various stress conditions like low temperature growth, and adaptation to high temperatures or high salinity, e.g. for Arg1p, Sti1p and Pdc1p. Our data on the differential regulation of the two genomes in these hybrid strains may have important industrial implications for strain improvement and strain protection. |
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Keywords: | Differential stress regulation Lager yeast Saccharomyces |
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