Somatic embryogenesis and plant regeneration in zygotic embryo cultures of balloon flower |
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Authors: | Kim Suk W Liu Jang R |
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Institution: | (1) Genetic Resources Center, Gene Bank, Korea;(2) Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Taejon, 305-600, Korea requests for offprints |
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Abstract: | Mature zygotic embryos of balloon flower (Platycodon grandiflorum) formed embryogenic calluses at a frequency of 43% when cultured on Murashige and Skoog medium supplemented with 4.52 μM
2,4-dichlorophenoxyacetic acid (2,4-D). Cell suspension cultures were established from embryogenic calluses using MS liquid
medium with 4.52 μM 2,4-D. Following transfer to solid MS basal medium, cell suspension cultures gave rise to somatic embryos,
which then developed into plantlets. Plantlets were transplanted to potting soil and grown to maturity.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Campanulaceae Platycodon grandiflorum somatic embryo suspension culture |
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