Abstract: | We investigated the effect of short-term exposure to cadmium and 2,4-dichlorophenoxy acetic acid on the digestive physiology
of Daphnia magna and the consequences for the bioenergetics of the organism. In both cases, ingestion was more drastically
reduced compared to digestive enzyme activity. Furthermore a differential shift in catabolism was noted: in general polysaccharidases
were less affected than the enzymes responsible for protein and lipid digestion. Comparison of the ‘1 h in vivo fluorescence’
criterion (Janssen & Persoone, 1993) with the ingestion and digestive enzyme activity revealed that this rapid screening assay
should be considered as a quantification of ingestion inhibition rather than a methodology assessing digestive enzyme inhibition.
This revised version was published online in July 2006 with corrections to the Cover Date. |