Method for determination of sodium, potassium, calcium, magnesium, chloride, and phosphate in the rat choroid plexus by flame atomic absorption and visible spectroscopy |
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| Authors: | V A Murphy |
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| Affiliation: | 1. Department of Medical Biochemistry, Max F. Perutz Laboratories, Medical University of Vienna, Vienna, Austria;2. Department of Neurology, Technische Universität Dresden, German Centre for Neurodegenerative Diseases (DZNE), Dresden, Germany;1. Instituto Geológico y Minero de España, Seville, Spain;2. Centro de Investigación en Química Sostenible — CIQSO, Universidad de Huelva, Campus El Carmen, Avda Fuerzas Armadas s/n, 21071 Huelva, Spain;3. Grupo/Centro de Hidrogeología, Departamento de Geología, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, Spain |
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| Abstract: | A rapid simple technique for the measurement of Na+, K+, Mg2+, Ca2+, PO4(3-), and Cl- was developed to analyze ion contents in the choroid plexus of the rat. The technique involves digestion in piperidine, precipitation of proteins with HClO4, and analysis of Na+, K+, Ca2+, and Mg2+ by atomic absorption spectroscopy and Cl- and PO4(3-) by visible spectroscopy. The coefficient of variation for the measurement of eight replicates was 1-3% for all ions. Analysis of choroid plexuses from eight rats yielded coefficients of variation of about 6% and the values for Na+, K+, and Cl- compared favorably to previous works. The analytical procedure described in this paper allows the determination of six major physiologic ions in rat choroid plexus (4 mg wet wt). |
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