Stabilization of proteins immobilized on Sepharose from leakage by glutaraldehyde crosslinking |
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Authors: | R Kowal R G Parsons |
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Institution: | Biological Markers Program, NCl Frederick Cancer Research Center, Frederick, Maryland 21701 USA |
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Abstract: | A technique has been developed to prevent leakage of proteins immobilized on Sepharose without destroying their biological functions. This involves the use of glutaraldehyde at concentrations ranging from 0.015 to 0.25% () to crosslink proteins, which had been coupled to Sepharose by conventional methods. Glutaraldehyde crosslinking decreases immuno-globulin G leakage from Sepharose-immunoadsorbents to undetectable levels without noticeably affecting antigen-binding activity and reduces leakage of lactoperoxidase from solid-phase lactoperoxidase with only a moderate reduction of enzymatic activity. |
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