Atrial natriuretic factor: atrial conversion of high to low molecular weight forms |
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Authors: | N C Trippodo R D Ghai A A MacPhee F E Cole |
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Affiliation: | 1. Division of Research, Alton Ochsner Medical Foundation New Orleans, Louisiana, USA;2. Research Department, Pharmaceutical Division Ciba-Geigy Corporation, Summit, New Jersey, USA |
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Abstract: | The atrial natriuretic factor elutes by gel filtration in high and low molecular weight fractions. Extraction and elution of rat atria in 1.0 M acetic acid yielded a predominance of the high molecular weight form(s); whereas when these procedures were carried out in 0.1 M acetic acid, there was a predominance of the low molecular weight forms. When partially purified high molecular weight natriuretic activity was eluted in 0.1 M acetic acid, the high molecular weight form(s) remained intact. When partially purified high molecular weight natriuretic activity was mixed with crude atrial extract in 0.1 M acetic acid, there was an apparent conversion to the low molecular weight forms. Extraction of rat atria in boiling 0.1 M acetic acid blocked this conversion. It is concluded that rat atria contain a heat labile factor that converts high molecular weight natriuretic activity to the low molecular weight forms. |
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Keywords: | K |
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