Identification of RAPD markers linked to a locus involved in quantitative resistance to TYLCV in tomato by bulked segregant analysis |
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| Authors: | V Chagué J C Mercier M Guénard A de Courcel F Vedel |
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| Institution: | (1) Institut de Biotechnologie des Plantes, Batiment 630, URA CNRS 1128, Université Paris-Sud, 91405 Orsay cedex, France, FR;(2) Clause Semences, Station de Recherche de Saint Rémy, Le Mas St Pierre, 13210 St Rémy de Provence, France, FR;(3) Clause Semences, 1 Avenue Lucien Clause, 91220 Brétigny sur Orge, France, FR |
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| Abstract: | In tomato, Bulked Segregant Analysis was used to identify random amplified polymorphic DNA (RAPD) markers linked to a quantitative
trait locus (QTL) involved in the resistance to the Tomato Yellow Leaf Curl Virus. F4 lines were distributed into two pools, each consisting of the most resistant and of the most susceptible individuals, respectively.
Both pools were screened using 600 random primers. Four RAPD markers were found to be linked to a QTL responsible for up to
27.7% of the resistance. These markers, localized in the same linkage group within a distance of 17.3 cM, were mapped to chromosome
6 on the tomato RFLP map.
Received: 21 August 1996 / Accepted: 4 April 1997 |
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| Keywords: | Bulked Segregant Analysis Marker-assisted selection QTL RAPD Tomato Yellow Leaf Curl Virus |
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