Genetic markers and sample types for pedigree reconstruction in Grevy's zebra (Equus grevyi) |
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| Institution: | 1. Wildlife DNA Services Limited, Science Campus, University of Wales, Bangor, LL57 2UW, United Kingdom;2. Marwell Preservation Trust Ltd., Colden Common, Winchester, SO21 1JH, United Kingdom;1. Department of Ecology and Evolutionary Biology, 106A Guyot Hall, Princeton University, Princeton, NJ 08544, USA;2. Department of Biological Sciences, 2119 Derring Hall (4020A), Virginia Polytechnic and State University, Blacksburg, VA, 24061, USA;1. North of England Zoological Society, Chester Zoo, Chester CH2 1LH, UK;2. University of Glasgow, Glasgow G12 8QQ, UK;3. University of California Davis, CA 95616, USA;4. Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UK;5. Smithsonian Conservation Biology Institute, Center for Species Survival, VA 22630, USA;1. Institut für Anthropologie, Johannes Gutenberg-Universität Mainz, Anselm-Franz-von-Bentzel-Weg 7, 55099 Mainz, Germany;2. Département de Biologie Animale, Faculté des Sciences, Université d’Antananarivo, BP 906, Antananarivo 101, Madagascar;1. Research and Scientific Services, National Zoological Garden, South African National Biodiversity Institute, 232 Boom Street, Pretoria, 0001, South Africa;2. Department of Genetics, University of the Free State, 205 Nelson Mandela Drive, Bloemfontein, 9300, South Africa;3. Epidemiology, Parasites and Vectors, Agricultural Research Council, Onderstepoort Veterinary Research, Onderstepoort, 0110, South Africa |
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| Abstract: | Effective captive population management relies on maintaining accurate pedigree records in order to direct breeding programmes and maximise conservation of genetic diversity. Where familial relationships or individual identities are unknown, it is now possible to recover this information via genetic analysis. We describe the first application of DNA profiling to reconstruct family groups of the Grevy's zebra (Equus grevyi), in order to identify three unknown stallions. To examine the potential for using non-invasive sampling techniques, DNA recovered from faeces, hair and blood was compared. Microsatellite loci derived from horses were tested for their utility in Grevy's zebra and a set of loci were subsequently employed to successfully identify the three animals via parental exclusion. The study demonstrates the relative ease with which DNA profiling can now be applied to captive management and highlights methods of genetic sampling that minimise stress to the animal and reduce sampling costs. |
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