| 抗虫基因转化优良籼型保持系D297B的研究 |
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| 引用本文： | 马炳田,王玲霞,李平,朱祯,周开达.抗虫基因转化优良籼型保持系D297B的研究[J].实验生物学报,2003,36(4):249-254. |
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| 作者姓名： | 马炳田 王玲霞 李平 朱祯 周开达 |
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| 作者单位： | [1]四川农业大学水稻研究所，成都温江611130 [2]四川省农业生物技术工程研究中心，成都温江611130 [3]中国科学院遗传与发育生物学研究所，北京100101 |
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| 摘 要： | 本文报道了用基因枪法将雪花莲外源凝集素基因(gna)转移到优良籼型杂交稻保持系D297B中。通过PCR、Southern blotting和Western blotting等分子检测方法证明，外源基因已整合到受体材料的基因组中并得到表达。蛋白活性测定结果显示转基因在受体中表达的蛋白具有生物活性。潮霉素抗性分析表明外源基因多数是以单位点形式整合并以3：1方式遗传。另外，PCR证据也说明抗虫基因与选择标记基因基本上是共整合和共遗传的。
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| 关 键 词： | 籼稻 D297B 遗传转化 基因枪转化法 雪花莲外源凝集素基因 抗虫性 |
Study on transgenic elite indica container line D297B containing foreign insect-resistant gene gna] |
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| Bing Tian Ma,Ling Xia Wang,Ping Li,Zhen Zhu,Kai Da Zhou.Study on transgenic elite indica container line D297B containing foreign insect-resistant gene gna][J].Acta Biologiae Experimentalis Sinica,2003,36(4):249-254. |
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| Authors: | Bing Tian Ma Ling Xia Wang Ping Li Zhen Zhu Kai Da Zhou |
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| Institution: | Rice Research Institute of Sichuan Agricultural University, Sichuan Agricultural Biotechnology Engineering Research Center, Chengdu 611130, China. |
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| Abstract: | It was reported in this article that the transgenic elite indica container line D297B containing snowdrop lectin (Galanthus nivalis agglutinin, GNA) gene gna was obtained by biolisties. PCR, Southern blotting and Western blotting indicated that the transgenes were integrated into the genome of D297B and expressed in transgenic plants. Analysis of protein activity showed that product of transgene had activity of agglutinin. Resistance of transgenic seeds to Hygromycin B suggested that the transgenes were integrated in a single locus and inherited according to the pattern of 3:1 in most of transgenic plants. In addition, the PCR analysis revealed that the insect-resistant gene gna and selective marker gene hpt were co-integrated and co-inherited. |
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