Hypoxia regulates human mast cell adhesion to fibronectin via the PI3K/AKT signaling pathway |
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| Authors: | Joanna Pastwińska Aurelia Walczak-Drzewiecka Magdalena ?ukasiak Marcin Ratajewski |
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| Institution: | 1. Laboratory of Cellular Immunology, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland;2. Department of Experimental Immunology, Medical University of Lodz, Lodz, Poland;3. Laboratory of Epigenetics, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland |
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| Abstract: | ABSTRACTA decrease in oxygen concentration is a hallmark of inflammatory reactions resulting from infection or homeostasis disorders. Mast cells interact with extracellular matrix and other cells by adhesion receptors. We investigated the effect of hypoxia on integrin-mediated mast cell adhesion to fibronectin. We found that it was mediated by the α5/β1 receptor and that hypoxia significantly upregulated this process. Hypoxia-mediated increases in mast cell adhesion occurred without increased surface expression of integrins, suggesting regulation by inside-out integrin signaling. Hypoxia also mediated an increase in phosphorylation of Akt, and PI3’kinase inhibitors abolished hypoxia-mediated mast cell adhesion. Hypoxia upregulates the function of integrin receptors by PI3’ kinase-dependent signaling. This process might be important for the location of mast cells at inflammatory sites |
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| Keywords: | Mast cells LAD2 adhesion fibronectin integrin α5β1 hypoxia SCF PI3K AKT wortmannin |
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