Precocious induction of arginase in primary cultures of fetal rat hepatocytes |
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Authors: | Annie Husson Mimouna Bouazza Catherine Buquet Rene Vaillant |
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Institution: | (1) Laboratoire d'Endocrinologie ERA 891, Faculté des Sciences et des Techniques, 76130 Mont-Saint-Aignan, France |
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Abstract: | Summary Fetal rat hepatocytes were isolated and cultured in primary culture to investigate activity changes of arginase under defined
conditions. In hormone-free medium, cultured cells maintained the enzyme activity at levels equal to that of freshly isolated
cells for at least 4 d. Arginase activity could be induced by dexamethasone in hepatocytes isolated from 16.5-d-old fetuses
although cells were competent to respond to glucagon only at the stage of 18.5 d. The combination of the two hormones induced
greater levels of arginase activity than the individual compounds. These findings indicate that glucocorticoid and glucagon
receptors appear early and sequentially before birth and reveal that cultured fetal hepatocytes provide a suitable system
for the investigation of the role of hormones in the initiation of enzyme synthesis.
This work was supported by the Institut National Scientifique et de la Recherche Médicale through Grant 85.80.117. |
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Keywords: | arginase dexamethasone glucagon fetal hepatocytes |
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