A new method for the preperative and analytical electrophoresis of cells |
| |
Authors: | Anna Wilk Kinga Rośkowicz Włodzimierz Korohoda |
| |
Institution: | (1) Department of Cell Biology, Faculty of Biotechnology, Jagiellonian University, ul. Gronostajowa 7, 30-378 Kraków, Poland |
| |
Abstract: | In this paper, a new method is described for the horizontal electrophoresis of cells on a density cushion under near-isopycnic
conditions. When cell sedimentation is minimized, the electrophoresis of red blood cells (RBC) used as model cells within
an anti-convective porous matrix (with pores over 300 μm in diameter) was capable of separating a mixture of human and chicken
RBC according to their electrophoretic mobilities. Samples taken from the separated RBC bands show over 90% purity for each
species. The simultaneous electrophoresis of several RBC samples carried out under identical conditions permitted the use
of comparative data based on the electrophoretic mobility of cells which differ in their surface properties. We believe that
this relatively simple system, in which cell sedimentation and convection are minimized, has the potential to be modified
and adapted for the separation of other cell types/organelles. |
| |
Keywords: | Cell electrophoresis Cell separation Cell surface Red blood cells |
本文献已被 PubMed SpringerLink 等数据库收录! |
|