Hiding behind hydrophobicity. Transmembrane segments in mass spectrometry |
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Authors: | Eichacker Lutz A Granvogl Bernhard Mirus Oliver Müller Bernd Christian Miess Christian Schleiff Enrico |
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Institution: | Department für Biologie I, Ludwig-Maximilians Universit?t München, Menzinger Strasse 67, 80368 München, Germany. eichacker@lrz.uni-muenchen.de |
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Abstract: | Proteomics of membrane proteins is essential for the understanding of cellular function. However, mass spectrometric analysis of membrane proteomes has been less successful than the proteomic determination of soluble proteins. To elucidate the mystery of transmembrane proteins in mass spectrometry, we present a detailed statistical analysis of experimental data derived from chloroplast membranes. This approach was further accomplished by the analysis of the Arabidopsis thaliana proteome after in silico digestion. We demonstrate that both the length and the hydrophobicity of the proteolytic fragments containing transmembrane segments are major determinants for detection by mass spectrometry. Based on a comparative analysis, we discuss possibilities to overcome the problem and provide possible protocols to shift the hydrophobicity of transmembrane segment-containing peptides to facilitate their detection. |
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