Semi-Quantification of GAX and GA4 in Male-Sterile Anthers of Rice by Radioimmunoassay |
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Authors: | Nakajima Masatoshi; Yamaguchi Isomaro; Kizawa Satoru; Murofushi Noboru; Takahashi Nobutaka |
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Institution: | Department of Agricultural Chemistry, The University of Tokyo Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113 Japan |
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Abstract: | The levels of endogenous GA, and GA4 in the anthers of a standardcultivar and the corresponding male-sterile, single gene mutantof rice, Oryza saliva L. (Japonica), were measured by radioimmunoassayusing GA4-specific antiserum that showed highly specific immunoreactivitywith GA, and GA4. The levels of these GAs in the anthers ofthe male-sterile mutant were about one-fifth to one-sixth ofthose in the normal cultivar, suggesting a correlation betweenthe endogenous levels of these GAs and the male sterility.
2Present address: Department of Agricultural Chemistry, UtsunomiyaUniversity, Mine-machi 350, Utsunomiya, 321 Japan (Received August 8, 1990; Accepted March 7, 1991) |
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