| 过氧化心肌线粒体产生的脂类自由基及（－）－EGCG的抑制作用 |
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| 引用本文： | 沈生荣,杨贤强,杨法军,赵保路,忻文娟. 过氧化心肌线粒体产生的脂类自由基及（－）－EGCG的抑制作用[J]. Acta biochimica et biophysica Sinica, 1995, 0(1) |
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| 作者姓名： | 沈生荣 杨贤强 杨法军 赵保路 忻文娟 |
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| 作者单位： | 浙江农业大学茶叶研究所，中国科学院生物物理研究所 |
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| 基金项目： | 国家自然科学基金,中国科学院“八五”重点资助 |
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| 摘 要： | 本文利用ＥＳＲ技术研究了心肌线粒体酶修饰下的脂质过氧化和脂类自由基，以及（－）－ＥＧＣＧ的抑制作用。结果表明：４－ＰＯＢＮ能捕集ｌｉｐｏｘｙｇｅｎｇｓｅ诱发心肌线粒体产生的自由基，得到６条线谱的脂类自由基和４条线谱捕集物，（－）－ＥＧＣＧ对该体系中使用的１ｉｐｏｘｙｅｎａｓｅ活性无影响，对所产生的自由基有明显的清除作用，并呈量效关系。对脂质过氧化的抑制作用，在本试验浓度范围内随浓度增加变化不大，最大抑制率约２０％。
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| 关 键 词： | 自由基，脂质过氧化，心肌线粒体，电子自旋共振，天然抗氧化剂 |
Lipids Free Radicals Produced by Myocardial Mitochondrla during Lipid Peroxidation and the Inbibitory Effect of (-)-EGCG |
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| SHEN Sheng-Rong and YANG Xian-Qiang. Lipids Free Radicals Produced by Myocardial Mitochondrla during Lipid Peroxidation and the Inbibitory Effect of (-)-EGCG[J]. Acta biochimica et biophysica Sinica, 1995, 0(1) |
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| Authors: | SHEN Sheng-Rong and YANG Xian-Qiang |
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| Abstract: | The lipid peroxidation of myooardial mitochondria induced by lipoxygenase and lipid radicals,and the inhibitory effects of (-)-epigalloeatechin gallate [(-)-EGCG] were studied by uaing the electron spin-trapping resonance technique. The results showed that 4-POBN could trap free radicals produced by lipoxygenase in myocardial mitoehondria and got a ESR apectrum including six lines and four lines peaks. (-)-EGOG had no effect on the activity of lipoxygenase,but scavenged the lipid radioals of the Bystem and showed doseefficiency relationship.In addition, (-)-EGCG could inhibit lipid Peroaldation,but, the inhibitory effects did not change with the increasing eoncentrations of (-)-EGCG used among in our experiment.The inhibitory ratio was about 21%. |
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| Keywords: | Free radicals Lipid peroridation Myocardial mitocnondria Electron spin resonance Natural antioxidant |
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