Lactase Production from Lactobacillus acidophilus |
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Authors: | S K Akolkar A Sajgure S S Lele |
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Institution: | (1) Food and Fermentation Technology Department, University Institute of Chemical Technology, Matunga, Mumbai, 400 019, India |
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Abstract: | Summary A lactobacillus strain isolated from fermented Ragi (Eleusine coracana Gaertn.) was characterized as Lactobacillus acidophilus. The isolate was found to be homofermentative, slime-forming and a lactase (β-galactosidase) producer. Production, recovery, characterization and performance of lactase were studied at laboratory scale
from 100 ml to 5 l under stationary and stirred conditions. 1.5% lactose was found to be the best carbon source for lactase
production. The lactose content could be reduced to 0.75% by supplementing with 1% ragi, thus making the media economically
more attractive. A 6.5-fold increase (5400 U ml−1) was achieved on scale-up. Performance of the lactase obtained from this strain was found to be slightly better than the
commercial lactase produced by Kluyveromyces lactis. |
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Keywords: | Eleusine coracana lactase Lactobacillus acidophilus scale-up yield optimization |
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