首页 | 本学科首页   官方微博 | 高级检索  
   检索      


The embryonic axis controls lipid catabolism in cotyledons of apple seeds during germination
Authors:Renata Bogatek  Bo&#;ena Zarska-Maciejewska  Irena Si&#;ska  Stanislaw Lewak
Institution:Inst. of Botany, Univ. of Warsaw, Krakowskie Przedmie?cie 26–28, PL-00–927 Warszawa, Poland;.
Abstract:Activities of alkaline lipase (AlkL, EC 3.1.1.3), isocitrate lyase (ICL, EC 4.1.3.1), glucose 6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) and pyruvate kinase (PK, EC 2.7.1.40) were determined in embryos of apple ( Malus domestica Borb. cv. Antonówka) during culture in darkness or at 12 h photoperiod; in both cases either in the presence of gibberellin A3 (GA3) or AMO 1618 (inhibitor of GA synthesis). AlkL and ICL were stimulated by light and GA3; light stimulation was reversed by AMO. G6PDH and PK were not affected by culture conditions. Almost all the activity of all enzymes was found in the cotyledons; only PK was distributed between axis and cotyledons. GA-like activity was found almost exclusively in the embryo axis. Cultured isolated cotyledons lost their sensitivity to light and AMO, but AlkL and ICL were still stimulated by GA3. Translocation of GA from axis to cotyledons during the culture of embryos is postulated.
Keywords:Apple  embryo  gibberellin  gluconeogenesis  light  lipolysis              Malus domestica            pentose phosphate path
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号