A novel technique for the enumeration of bacteriophage from water |
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| Authors: | R.N. Purdy B.N. Dancer M.J. Day D.J. Stickler |
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| Affiliation: | Department of Applied Biology, University of Wales Institute of Science and Technology, King Edward VII Avenue, Cardiff CF1 3NU, U.K. |
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| Abstract: | Abstract A novel method has been developed for concentrating and enumerating bacteriophages in water. Host bacterial cells are added to water samples to adsorb phage and then the infected bacteria are collected by centrifugation at 4000 rev./min for 30 min. The pellets are resuspended in small volumes and assayed for phages using the agar-overlay method. This procedure was more successful than three established techniques in recovering Bacillus phages from seeded samples of tap water. It also gave efficient recovery of phages from samples of river, lake and sea-water. |
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| Keywords: | Bacteriophage Bacillus water enumeration host adsorption |
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