Somatic embryogenesis in protoplast derived calli of cultivated jute,Corchorus capsularis L. |
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Authors: | Thakurdas Saha S K Sen |
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Institution: | (1) Plant Molecular & Cellular Genetics, Bose Institute, Centenary Building, P1/12, C.I.T. Scheme VII-M, 700054 Calcutta, India |
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Abstract: | Protoplasts were isolated from cotyledon, hypocotyl and mesophyll cells of Corchorus capsularis L., a major fibre crop, by one step enzyme digestion method. They were further cultured successfully on modified KM-8p (Kao and Michayluk, 1975) medium to form microcalli. The required cultural conditions could be used to achieve 34% to 78% plating efficiency, depending upon the source of protoplasts. Hypocotyl protoplasts gave the highest plating efficiency. On transfer to regeneration medium, somatic embryos developed at high frequency. The present success is a significant step forward in the development of meaningful plant cell culture methods for application in jute.Abbreviations B5
Gamborg et al., 1968
- MS
Murashige and Skoog, 1962
- SH
Schenk and Hildebrandt, 1972
- Ad-SO4
Adenine sulphate
- BAP
6-benzylaminopurine
- NAA
1-naphthalene acetic acid
- GA3
Gibberellic acid
- 2,4-D
2,4-dichlorophenoxy acetic acid
- Kn
Kinetin
- IBA
Indole-3-butyric acid |
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Keywords: | |
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