Construction of random sheared fosmid library from Chinese cabbage and its use for Brassica rapa genome sequencing project |
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| Authors: | Tae-Ho Park Beom-Seok Park |
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| Institution: | Tae-Ho Park~(a,b,1),Beom-Seok Park~(a,*,Jin-A Kim~a,Joon Ki Hong~a,Mina Jin~a,Young-Joo Seol~a,Jeong-Hwan Mun~a a Genomics and Functional Bio-Material Division,National Academy of Agricultural Science,Rural Development Administration,Suwon 441-707,Republic of Korea b The Department of Horticultural Science,Daegu University,Gyeongsan 712-714,Republic of Korea |
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| Abstract: | As a part of the Multinational Genome Sequencing Project of Brassica rapa,linkage group R9 and R3 were sequenced using a bacterial artificial chromosome(BAC) by BAC strategy.The current physical contigs are expected to cover approximately 90%euchromatins of both chromosomes.As the project progresses,BAC selection for sequence extension becomes more limited because BAC libraries are restriction enzyme-specific.To support the project,a random sheared fosmid library was constructed.The library consists of 9753... |
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| Keywords: | Brassica rapa Chinese cabbage Fosmid library Genome sequencing Physical contig |
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