Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast |
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| Authors: | Eva García-Ruiz Diana Maté Antonio Ballesteros Angel T Martinez Miguel Alcalde |
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| Institution: | 1.Department of Biocatalysis,Institute of Catalysis, CSIC,Madrid,Spain;2.Centro de Investigaciones Biológicas,CSIC,Madrid,Spain |
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| Abstract: | Background In the picture of a laboratory evolution experiment, to improve the thermostability whilst maintaining the activity requires
of suitable procedures to generate diversity in combination with robust high-throughput protocols. The current work describes
how to achieve this goal by engineering ligninolytic oxidoreductases (a high-redox potential laccase -HRPL- and a versatile
peroxidase, -VP-) functionally expressed in Saccharomyces cerevisiae. |
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