Optimization of pyrene oxidation by Penicillium janthinellum using response-surface methodology |
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Authors: | L A Launen L J Pinto M M Moore |
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Institution: | (1) Simon Fraser University, Department of Biological Sciences, 8888 University Drive, Burnaby, BC, V5A 1S6, Canada e-mail: mmoore@sfu.ca Tel.: +1-604-291-3441 Fax: +1-604-291-3496, CA |
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Abstract: | At present, there is little information on the optimization of the degradation of polycyclic aromatic hydrocarbons (PAH)
by deuteromycete filamentous fungi, a reaction catalyzed by cytochrome P450 monooxygenases. We utilized response-surface methodology to determine the optimal growth conditions for the oxidation
of the PAH pyrene by Penicillium janthinellum SFU403, with respect to the variables glucose concentration, nitrate concentration and bioconversion time. Models were derived
for the relationship between the variables tested and the level of the pyrene oxidation products, 1-pyrenol (1-PY) and pyrenequinones
(PQ). Production of 1-PY and PQ were optimized by the same glucose and nitrate concentrations: 2.5% glucose and 1.5% sodium
nitrate. The optimized 1-PY and PQ bioconversion times were 71 h and 73 h respectively. These conditions improved the yield
of 1-PY by fivefold and PQ were more than 100-fold higher than the baseline levels obtained in this study. The optimized PQ
yield represented 95% of the initial pyrene, thus the total optimised pyrene bioconversion to 1-PY and PQ was approximately
100%. Concentrations of glucose exceeding 4.0% repressed pyrene hydroxylation. Pyrene hydroxylation occurred almost exclusively
during the deceleration phase of culture growth.
Received: 20 July 1998 / Received revision: 7 December 1998 / Accepted: 10 January 1999 |
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