Intracellular location of cytochrome oxidase active in vivo in the cyanophytes,Synechocystis sp. PCC6714 andAnacystis nidulans Tx20 and R2, grown under various conditions |
| |
Authors: | K Ohki Y Fujita |
| |
Institution: | (1) Department of Marine Science, School of Marine Science and Technology, Tokai University, 3-20-1 Orido, Shimizu, 424 Sizuoka, Japan;(2) Department of Cell Biology, National Institute for Basic Biology, Okazaki, Aichi |
| |
Abstract: | Summary Intracellular location of cytochrome oxidase (cytoxidase) active in vivo was studied cytochemically with four strains of cyanophytes, using 3,3 -diaminobenzidine (DAB) oxidation. DAB was oxidized in the dark bySynechocystis sp. PCC6714 and two strains ofAnacystis nidulans (Tx20 and R2) grown under photoautotrophic, heterotrophic and salt-stressed conditions, respectively. Electron microscopic observations showed that DAB-oxidation in the dark occurred in the thylakoids, but was insignificant on or around the cytoplasmic membrane. However, deposition of DAB-oxidation product around the cytoplasmic membrane was observed with cells of the thylakoid-less cyanophyteGlaeobacter violaceus ATCC29082. All DAB oxidations observed with the four strains were inhibited completely by cyanide, the inhibitor of cyt-oxidase, but not by 3-amino-1,2,4-triazole, the inhibitor of peroxidase. The results show that (1) DAB was oxidized by the cyt-oxidase functioning in the respiratory system, and that (2) cyt-oxidase in thylakoids was active in vivo.Abbreviations AT
3-amino-1,2,4-triazole
- Cyt
oxidase cytochrome oxidase
- ETS
electron transport system
- DAB
3,3 -diaminobenzidine |
| |
Keywords: | Cyanophytes Cytochrome oxidase Electron transport DAB oxidation Respiration |
本文献已被 SpringerLink 等数据库收录! |
|