Characterization of a localized basophil hypersensitivity lesion in guinea pig conjunctiva |
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| Authors: | A H Cornell-Bell L E Hann K J Bloch M R Allansmith |
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| Affiliation: | 1. Eye Research Institute, Boston, Massachusetts 02114 U.S.A.;2. Departments of Ophthalmology and Medicine, Harvard Medical School, Boston, Massachusetts 02114 U.S.A.;3. The Clinical Immunology and Allergy Units, Massachusetts General Hospital, Boston, Massachusetts 02114 U.S.A.;1. Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Hefei 230027, China;2. Fujian Academy of Building Research, Fuzhou 350000, China;3. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China;1. School of Soil and Water Conservation, Beijing Forestry University, Key Laboratory of State Forestry Administration on Soil and Water Conservation, Beijing, 100083, China;2. Beijing Forestry University, Beijing Engineering Research Center of Soil and Water Conservation, Beijing, 100083, China;3. Beijing Forestry University, Engineering Research Center of Forestry Ecological Engineering, Ministry of Education, Beijing, 100083, China |
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| Abstract: | Basophils accumulate in response to antigen challenge in cutaneous basophil hypersensitivity (CBH) reactions. Two ocular diseases, vernal conjunctivitis and contact-lens-associated conjunctivitis, are also characterized by this histopathology. We have refined a model previously developed in guinea pig conjunctiva by precisely defining the site of antigen injection and correlating the site with the clinical and histologic changes. Guinea pigs were primed by an intradermal injection of keyhole limpet hemocyanin (KLH) in the flank and challenged (Day 6) by injection of a small bolus of KLH just under the conjunctival epithelium. Twenty-four hours later histologic examination showed a perivascular infiltrate of inflammatory cells containing large numbers of basophils. Eosinophils, neutrophils, macrophages, and mast cells were also seen. Serial sections of the reaction site showed discrete boundaries. At all sites examined in antigen-challenged tissues, there were significantly more basophils than in control-injected conjunctiva. Insertion of a sterile needle or injection of PBS or KLH into normal conjunctiva induced a significant increase in neutrophils and some macrophages. Injection of graded doses of antigen into the conjunctiva of primed animals, resulted in a dose-dependent increase in basophils up to 50 micrograms KLH (optimal dose). |
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