A simplified purification and some properties of ribulose 1,5-diphosphate carboxylase from barley |
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Authors: | Kleinkopf G E Huffaker R C Matheson A |
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Institution: | Department of Agronomy and Range Science and Department of Veterinary Microbiology, University of California, Davis, California 95616. |
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Abstract: | A rapid procedure was developed for purifying ribulose 1,5-diphosphate carboxylase from barley leaves. After (NH4)2SO4 fractionation, the unique sedimentation properties of the enzyme were exploited to effect a single step purification to 90% homogeneity. High speed centrifugation pelleted the enzyme with complete recovery of activity. Residual impurities were then removed by diethylaminoethyl cellulose chromatography and density gradient centrifugation. The purified protein exhibited size heterogeneity due to polymerization. The polymerization products were enzymatically active aggregates of ribulose 1,5-diphosphate carboxylase and were precipitated by an antibody specific for the enzyme. |
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