Extracellular Matrix of Porcine Pericardium: Biochemistry and Collagen Architecture |
| |
Authors: | Antonella Sachsida Braga-Vilela Edson Rosa Pimentel Sergio Marangoni Marcos Hikari Toyama Benedicto de Campos Vidal |
| |
Institution: | Department of Biologic Sciences, UNIFAL, Rua Gabriel Monteiro Silva 714, 37 130 000, Alfenas, Minas Gerais, Brazil. antonella@unifal-mg.edu.br |
| |
Abstract: | Pericardial tissue has been used to construct bioprostheses employed in the repair of different kinds of injuries, mostly
cardiac. However, calcification and mechanical failure have been the main causes of the limited durability of cardiac bioprostheses
constructed with bovine pericardium. In the course of this work, a study was conducted on porcine fibrous pericardium, its
microscopic structure and biochemical nature. The general morphology and architecture of collagen were studied under conventional
light and polarized light microscopy. The biochemical study of the pericardial matrix was conducted according to the following
procedures: swelling test, hydroxyproline and collagen dosage, quantification of amino acids in soluble collagen, component
extraction of the extracellular matrix of the right and left ventral regions of pericardium with different molarities of guanidine
chloride, protein and glycosaminoglycan (GAG) dosage, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and total
GAG analysis. Microscopic analysis showed collagen fibers arranged in multidirectionally oriented layers forming a closely
knit web, with a larger number of fibers obliquely oriented, initiating at the lower central region toward the upper left
lateral relative to the heart. No qualitative differences were found between proteins extracted from the right and left regions.
Likewise, no differences were found between fresh and frozen material. Protein dosages from left frontal and right frontal
pericardium regions showed no significant differences. The quantities of extracted GAGs were too small for detection by the
method used. Enzymatic digestion and electrophoretic analysis showed that the GAG found is possibly dermatan sulfate. The
proteoglycan showed a running standard very similar to the small proteoglycan decorin. |
| |
Keywords: | Extracellular matrix Porcine pericardium Collagen Proteoglycan glycosaminoglycan Bioprosthesis |
本文献已被 PubMed SpringerLink 等数据库收录! |
|