Erythroid differentiation factor stimulates hydrolysis of polyphosphoinositide in Friend erythroleukemia cells |
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Authors: | H Shibata E Ogata Y Etoh H Shibai I Kojima |
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Affiliation: | Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton, U.K. |
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Abstract: | We examined an early action of erythroid differentiation factor (EDF), a polypeptide which induces differentiation of Friend murine erythroleukemia (MEL) cells. (Eto et al., Biochem. Biophys. Res. Commun. 142: 1095-1103, 1987). In MEL cells, EDF caused a rapid and transient increase in cytoplasmic concentration of free calcium, [Ca2+]c. EDF increased [Ca2+]c even in the absence of extracellular calcium. When [3H]inositol-labeled MEL cells were incubated with EDF, EDF rapidly increased radioactivity in inositol trisphosphate, bisphosphate and monophosphate. EDF also increased [3H] diacylglycerol in [3H]arachidonate-labeled MEL cells. These results indicate that EDF increases [Ca2+]c by stimulating hydrolysis of polyphosphoinositide. |
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