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Optimization of an ethanol production medium in very high gravity fermentation
Authors:Fan-Qiang Wang  Cui-Juan Gao  Chun-Yu Yang  Ping Xu
Affiliation:(1) State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People’s Republic of China
Abstract:Concentrations of Mg2+, glycine, yeast extract, biotin, acetaldehyde and peptone were optimized by a uniform design process for ethanol production by Saccharomyces cerevisiae. Using non-linear step-wise regression analysis, a predictive mathematical model was established. Concentrations of Mg2+ and peptone were identified as the critical factors: 50 mM Mg2+ and 1.5% (w/v) peptone in the medium increased the final ethanol titre from 14.2% (v/v) to 17% (v/v) in 48 h.
Keywords:Ethanol  Fermentation  High gravity brewing   Saccharomyces cerevisiae
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