Intracellular pH and free calcium changes in single cells using quene 1 and quin 2 probes and fluorescence microscopy |
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| Authors: | J Rogers T R Hesketh G A Smith M A Beaven J C Metcalfe P Johnson P B Garland |
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| Affiliation: | 1. Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, England;2. Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, Scotland |
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| Abstract: | Photometric fluorescence microscopy has been used to measure intracellular pH (pHi) and free calcium concentrations [( Ca]i) in individual mouse thymocytes and 2H3 rat basophil leukaemic cells containing indicators for pH (quene 1) or calcium (quin 2). The pHi and [Ca]i measurements in individual 2H3 cells and mouse thymocytes and their responses to various stimuli were consistent with the corresponding data obtained from suspensions of these cells measured in a spectrofluorimeter. Photometric fluorescence microscopy of these indicators in individual cells provides a sensitive and fast method of following pHi and [Ca]i responses in individual cells. |
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| Keywords: | Intracellular pH Thymocyte 2H3 cell Antigenic stimulation Fluorescence microscopy |
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