A substance in conditioned medium which enhances the growth of small numbers of chick embryo cells |
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| Authors: | H Rubin |
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| Affiliation: | 1. Laboratory of Molecular Brain Science, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, 739-8521, Japan;2. Department of Biochemistry, Asahikawa Medical University, Asahikawa, 078-8510, Japan;3. Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa, 761-0795, Japan;1. Department of Internal Medicine, School of Medicine, Khorshid Hospital, Isfahan University of Medical Science, Isfahan, Iran;2. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran;3. Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran;4. School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;5. Exir Azma Salam Iranian Institute, Research and Development Department, Tehran, Iran;6. Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;7. Autoimmune Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran |
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| Abstract: | Medium obtained from high concentrations of chick embryo cells contains a factor which enhances the growth of small numbers of these cells. The conditioned medium obtained from very crowded cultures inhibits cell growth when used at high concentrations, and enhances cell growth when used at low concentrations. The growth enhancing activity is quickly lost at elevated temperatures and can be removed from the medium by prolonged high speed centrifugation. No activity can be demonstrated in the pellet, but a fraction of the initial activity can be recovered from the material immediately overlying the pellet. It is concluded that the growth enhancing activity resides in a large molecule or in aggregated molecules. |
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