Induction of G- and R-banding in human chromosomes by the demethylating agent S-adenosyl-L-homocysteine. |
| |
Authors: | S F De Cabo C Sentís J Fernández-Piqueras |
| |
Affiliation: | Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain. |
| |
Abstract: | In this report we describe the procedure of growing human lymphocytes with the demethylating agent S-adenosyl-L-homocysteine (SAH). After this treatment, which is not toxic for cell survival, both R- and G-banding were obtained by new experimental procedures: R-bands have been directly demonstrated with the GC-specific fluorochrome chromomycin A3 without the necessity of any AT-specific counterstaining agent; simultaneous G-banding and active nucleolar organizer regions have been obtained by silver impregnation of chromosomes and subsequent Giemsa staining. These results suggest a possible relationship between local differences in DNA methylation and the determination of the banded chromosome structure. |
| |
Keywords: | |
|
|