富钙灵芝的深层培养

目的:研究灵芝深层发酵过程中钙对灵芝生长及钙的富集作用的影响。方法:用重氮化法将ABA分别与牛血清白蛋白(BSA)、卵清白蛋白(OVA)连结,得到ABA的免疫抗原和包被抗原,并采用紫外全波长扫描和SDS-PAGE鉴定合成的抗原;以经过鉴定的抗原免疫白兔,制备出ABA的多克隆抗体;采用间接酶联免疫法对抗血清进行效价检测;通过离子交换层析法获得纯化的抗体。结果:当培养基中硝酸钙的添加量达到600mg/100g时,灵芝生长和富集钙的效果最好;添加量超过800mg/100g时,灵芝富集钙的效果明显降低。富钙灵芝中有机钙的含量约占总钙量的97.30%。结论:在培养基中添加低浓度的钙,对灵芝的生长和钙的富集有促进作用,为开发新型有机钙提供了依据。

Research on Deep Cultivation of Calcium-Enriched Gamoderma lucidum

Objective:To research the calcium application and influence on growth of Gamoderma lucidum during deep-submerged culture.Methods:ABA was conjugated to bovine serum album(BSA)and ovalbumin(OVA)via diazo coupling.The appraisal was identified by UV spectrometric scanning and SDS-PAGE.Rabbits were immunized with antigen to obtain the multi-clonal antibody against ABA.Antibody was identified by ELISA,and purified by ion exchange chromatography.Results:Based on the 600 mg/100 g calcium nitrate in the medium,the growth and calcium-enriched of G.lucidum was best.When adding amounts of calcium was about 800 mg/100 g,the efficiency of calcium-enriched obviously reduced.The organic calcium accounts for 97.3% of total calcium in the calcium-enriched G.lucidum.Conclusion:The results showed that adding some low density calcium in culture medium have a provoking effect in the growth of G.lucidum and enrichment of calcium,furthermore,which has supplied a fundamental to developing new kind of organic calcium.