Intracellular Ice Formation Is Affected by Cell Interactions |
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Authors: | J. P. Acker A. Larese H. Yang A. Petrenko L. E. McGann |
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Affiliation: | Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, T6G 2R8, Canada. |
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Abstract: | Cell-to-cell and cell-to-surface interactions are important to the structure and function of tissues. These interactions are also important determinants of low-temperature responses in tissues. Four in vitro models using hamster fibroblast cells in tissue culture were used to investigate the influence of cell-cell and cell-surface interactions on intracellular ice formation in these systems. The four models were: (a) single cells in suspension; (b) cells individually attached to glass with only cell-to-surface adhesion; (c) colonies of cells attached to glass with both cell-cell and cell-surface interactions; and (d) multicellular spheroids with extensive cell-cell contacts. Cryomicroscopy was used to monitor the prevalence and kinetics of intracellular ice formation after ice nucleation in the extracellular solution. The temperature for intracellular freezing in 50% of the cells was significantly affected by both cell-cell and cell-surface interactions. There was also evidence of intercellular nucleation through cell-cell interactions. The results indicate that cell-cell and cell-surface interactions play a significant role in the low-temperature response of tissue systems. |
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Keywords: | intracellular ice formation cell interactions cell adhesion tissue cryobiology cryomicroscopy |
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